Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(375-378)

EFFECT OF DIFFERENT GROWTH REGULATORS ON IN VITRO NODAL EXPLANT OF KINNOW MANDARIN IN NORTHERN RAJASTHAN, INDIA

CHANDRA GURNANI , VIKRAM KUMAR, SHINAM MUKHIJA, POONAM NARULA AND ABNI SHARMA

Abstract

Studies were initiated on the role of various growth regulators in regeneration process of Kinnow mandarin. In vitro nodal explants were cultured on MS media supplemented with different concentration of cytokinin(BAP, Kinetin), auxin (NAA, IAA, IBA) and combination of cytokinin with auxin to produce plantlets. Best shoot multiplication obtained on MS medium supplemented with BAP (1.5 mg/L) and NAA (0.5mg/L). Transfer of shoot regenerated plantlet inMS media supplemented with NAA and IBA for root proliferation. Highest percentage of roots obtained on MS media supplemented with NAA (1.5mg /L).

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