Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 1, 2008; Page No.(59-63)

STUDY OF METABOLISM OF A FLAVONE - CHRYSIN USING MICROBIAL CULTURES

M. VIDYAVATH1, D.R. KRISHNA K.V.S.R.G. PRASAD AND J. VIDYASAGAR

Abstract

The aim of the study was to develop a microbial model for synthesis of metabolite of a bioflavonoid, chrysin for further pharmacological and toxicological studies. The metabolite of chrysin in microbial cultures was identified, isolated and confirmed using fermentation techniques and HPLC followed by mass spectrometry. Among different organisms screened, Nocardia hydrocarbonoxydans showed an extra peak at 4.57 min. in HPLC compared with its controls indicating formation of a metabolite. The metabolite was further characterized by mass spectrometry and was found to be sulfate conjugate of acetyl, hydroxy chrysin in which sulfate conjugation was reported as one of the mammalian metabolic pathway of chrysin._Nocardia hydrocarbonoxydans could be able to metabolize chrysin by sulfate conjugation similar to human beings and also by acetylation and hydroxylation. This study has developed a model to produce metabolites of other drugs by acetyl and sulfate conjugation and aromatic hydroxylation by fermentation techniques.

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