Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 1, 2007; Page No.(19-26)

EFFECT OF ANTIMICROBIAL SUBSTANCES FROM BLIRKHOLDERIA MLILTIVORANS AND MICROBACTERILIM TESTACELIM AGAINST THE ASSOCIATED FUNGI WITH OIL PALM SEEDS

A. DIKIN, KAMARUZAMAN SIJAM, JUGAH KADIR AND IDRIS ABU SEMAN

Abstract

There were some species of fungi associated with oil palm seeds such as S. commune, Penicillium spp., Aspergillus spp., Botryodiplodia theobroinae, Trichodernia spp., and Fusarium solani. These fungi affected the quality of oil palm seeds in the market. Supernatant was separated from antimicrobial substances in the fermenting liquid medium which inoculated Burkholderia multivorans and Mic-robacterium testaceum affected the growth of fungi. The fermented liquid medium with basal medium neo peptone, lactose inoculated with B. inultivorans inhibited the growth of S. commune, Penicillium spp., Aspergillus spp., Botryodiplodia theobromae, and Fusarium sob-mi. It was the same as the fermented liquid medium with basal medium peptone, maltose inoculated with M. testaceum. Supernatant of antimicrobial substances from both species of bacteria did not significantly suppress the growth of Trichodema spp. Chromatograph of HPLC had profiles of supernatant from B. niultivorans containing phenylpyrrol. Supernatant from M. testaceum was content of aminopyrrol and two non-identified compounds. Bacterial extracts of B. niultivorans contained the active compounds such as isopyrrolnitrin, aminopyrrolnitrin, phenazine and eight non-distinguished peaks at different retention times. Bacterial extract of M. testaceum contained two non-distinguished peaks at different retention times.

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