THE EFFECT COMBINATION OF GROWTH REGULATOR 2,4D AND KINETIN ON CALLUS INDUCTION FROM ELEPHANTOPUS SCABER LEAFJunairiah, Ariana Sri Rejeki and Edy Setiti Wida Utami
Elephantopus scaber L. is a plant that has benefit as traditional medicine. The plant leaves can be used as medicine caused by their secondary metabolites such as flavonoid, phenol, saponin, tannin, steroid, terpene, and luteolin-7-glucoside. The production of secondary metabolites can be done by in vitro culture using culture media and appropriate growth regulators. This study aims to determine the effect of a combination of 2,4-D growth regulators and kinetin on callus induction time, percentage of explants forming callus, fresh weight, dry weight, and callus morphology. This study was a laboratory experimental study with a completely randomized design method with 8 treatments combined concentrations of growth regulators (D0,0K0,0; D0,5K1,5; D0,5K2,0; D1,0K1,0; D1,0K1,5; D1,5K0,5; D1,5K1,0; D2,0K1,0) and each treatment consisted of four replications. The data obtained was analyzed qualitatively and quantitatively. Qualitative data were obtained from callus morphological (color and texture) descriptions. Quantitative data obtained from callus induction time, the percentage of explants forming callus, fresh weight and dry weight of callus were statistically analyzed using SPSS 24 with a significance value of 0,05. Data analysis measured the effect of growth regulator 2,4-D concentrations and kinetin using the Kruskal Wallis test. Difference between treatment groups were tested using the Mann-Whitney test. The results showed that the D1,0K1,5 treatment group increased callus most significantly in comparison than another treatment groups, the average induction time of callus 10.8 days after planting. The D1,0K1,5 treatment group produced the highest average fresh weight of 119.5 mg and also the highest average dry weight was 78.9 mg. Callus morphology with compact texture and the yellowish color were produced at D0,5K2,0; D1,0K1,5 treatment group.