ASSESSMENT OF GENETIC DIVERSITY OF CERTAIN JASMINE ECOTYPES OF COASTAL KARNATAKA THROUGH RAPD ANALYSISChaitanya H.S., Nataraja S. and M. Krishnappa
Jasminum spp. Commonly-known as mallige in Kannada and this flower crop is popular because of its medicinal and aromatic character. There are about 2000 species recorded in the genus Jasminum and 40 species are recorded to be grown in India, out of which 20 species are cultivated in south India (Bhatacharjee, 1980). The coastal Karnataka, which comprises of three districts viz., Uttara Kannada, Udupi and Dakshina Kannada, three major ecotypes of jasmine are being cultivated namely Bhatkal mallige, Udupi mallige, and Mangalur mallige. Extraction of DNA was carried out from the six jasmine ecotypes viz., Bhatkal mallige (two locations- J1a, J1b), Udupi mallige (two locations- J2a, J2b), and Mangalur mallige (two locations- J3a, J3b) and the process of DNA fingerprinting were initiated through Random Amplified Polymorphic DNA (RAPD) using PCR. From the RAPD-PCR monomorphic and polymorphic bands were produced which represented the characteristics of the selected ecotypes of jasmine. Ten decamer primers were used for the purpose out of which 5 primers, three from OP-B, one from OP-C and one from OP-F series produced bright and clear bands which ranged from 100bp to 1500 bp. Overall 51 amplicons were detected, where 27 amplicons were polymorphic and 24 amplicons were monomorphic with a polymorphism of 47 per cent. Based on the results of DNA fingerprinting a dendrogram was drawn to study the genetic similarities and differences between the selected six jasmine ecotypes. The jasmine genotypes Bhatkal mallige and Udupi mallige showed a maximum similarity match of 86 percent. The investigation carried out revealed the extent of diversity among the cultivars of jasmine in coastal Karnataka.
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