STEM CELL FROM HUMAN EXFOLIATED DECIDUOUS TEETH (SHED) TRANSPLANTATION FOR ISCHAEMIC STROKE IN RATSYETTY RAMLI, SALIM HARRIS1, NANDINI PHALITA LAKSMI*1, MOHAMMAD KURNIAWAN1, MASAGUS ZAINURI, RATIHRINENDYA PUTRI, PUSPITAEKA WUYUNG AND SALEHA SUNGKAR
Ischemic stroke is a burden on health problems in Indonesia. The incidence of stroke reaches 15 million cases worldwide, with 5 million stroke survivors suffered permanent disability. The prevalence of stroke in Indonesia in 2013 was 12.1 per 1000 population. Therefore, the optimal method for stroke restoration is required. Neurorestoration with stem cell transplantation is a promising therapy that may improve functional outcomes in ischemic stroke. This research aims to observe the effects of Stem Cell from Human Exfoliated Deciduous Teeth (SHED) transplantation on functional outcomes and brain neuron death ratio in ischemic stroke rats model. Male Wistar rats were classified into 3 groups: normal group (n=5), MCA occlusion group (n=5), and MCA occlusion with SHED group (n=5). SHED transplantation performed 48 hours after MCAO, by intravenous injection with a dose of 2x106 cells/kg. Functional evaluation was conducted using Y Maze and cylinder test. Evaluation of the death neurons ratio in brain cortex area was done using Hematoxylin and Eosin (HE) staining. The functional evaluation with Y Maze and cylinder test was significantly improved in the stroke+SHED group compared with control (stroke) group on 14 days after MCAO (p < 0,05). There was a significantly reduced dead neuron cell using HE (p<0.001) on rats transplanted with SHED. SHED transplantation at 48 hours after stroke provides clinical improvement and reduction of dead neurons ratio in the brain of ischemic stroke rats model.
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