Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(710-718)

IMPROVED PERFORMANCE OF SEED COATING OF AM SPORE INOCULUM DEVELOPED THROUGH ROOT ORGAN CULTURE

R. VINU RADHA, P. MARIMUTHU AND K. KUMUTHA

Abstract

The immense root colonization potential of viable monoxenic species of Arbuscular mycorrhizal (AM), Glomus intraradices (Gi) established in Root Organ Culture (ROC) exhibited extensive spore load in a short span of time. These symbiotic fungi colonize the emerging young roots by an improved method of application through seed coating of AM spore inoculum. A cellulosic polymer used for seed coating facilitated easy adherence of these spores on maize seeds. The rate of AM (Gi) inoculum coated on seeds @ 50 g kg-1 seed had the best coating efficiency of 83.2 per cent with the mean AM root colonization of 57.2 per cent. Among various insecticides used in combined seed treatment, recommended dosage of Imidacloprid @ 5mL kg-1 seed coated prior to AM fungal coating recorded a minimum reduction in the root colonization, whereas seed coating of fungicides like Thiram and Carbendazim showed deleterious effect on root colonization when treated with AM inoculum. The pot culture study revealed that seed coating of Gi showed maximum of 62.7 per cent of AM root colonization. The combined seed coating of Imidacloprid @ 5 mL kg-1 seed and Gi @ 50 g kg-1 seed also recorded higher root colonization (55.0 per cent) but the conventional vermiculite based inoculum applied in soil showed only 52.0 per cent. Seed treatment of AM fungi developed through ROC was found to be better in improving the growth of plant.

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