Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(703-709)

OPTIMISATION OF NUTRITIONAL COMPOSITION AND CULTURAL CONDITIONS FOR THE PRODUCTION OF PECTINASE BY BACILLUS SUBTILIS

GEEDHU LOWRANCE, V.D. DEVI, ANJU JOSEPH AND A.K. VALSA

Abstract

A pectin degrading bacteria was isolated from the soil and was identified as Bacillus subtilis by staining, morphological, biochemical and molecular techniques. The optimum cultural conditions for the production of pectinase for the isolated organism were 30 oC and pH-6. Maximum enzyme production was at 72 hours of incubation. Optimum concentration of pectin was 1%, whereas those of natural substrates - orange peel and rice bran were 5%. Additional carbon sources - starch and glucose increased pectinase production, but additional nitrogen sources decreased pectinase production. Pectinase production by cells immobilized in 3% sodium alginate was higher than that of free cells. The immobilized cells could be used repeatedly for five cycles.

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