Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.( 683-687)

ENUMERATION OF ENDOPHYTIC FUNGI FROM EICHHORNIA CRASSIPES ROOT AND PRELIMINARY SCREENING FOR TANNASE ENZYME PRODUCTION

KALYANARAMAN RAJAGOPAL, SASIPRABHA P., SABARISH S., ARULMATHI R., MEENASHREE B. AND KATHIRAVAN G.

Abstract

Endophytic fungi were isolated from root tissue of Eichhornia crassipes a true hydrophytic plant. Three hundred root segments were sampled. Six different endophytic fungal species could be isolated. The endophytic fungi were belonging to Ascomycete, Basidiomycete and Hyphomycete viz Talaromyces aurantianum, Talaromyces minioluteus, Trametes versicolor, Trametes sp, Penicillium samsonii and Penicillium minioluteum. The endophytes were identified using ITS primers. Talaromyces aurantiacum, Trametes sp and Penicillium minioluteum showed higher colonization frequency. All the 6 endophytic fungi were tested for production of extracellular enzyme tannase. Talaromyces aurantianum, Trametes versicolor, Trametes sp, and Penicillium minioluteum produced tannase in plate assay.

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