Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

IMMUNOLOGICAL AND EPIDEMIOLOGICAL STUDY ON MOLLUSCUM CONTAGIOSUM VIRUS IN BABYLON PROVINCE

ZAYTOON A. ALKHAFAJI, HUSSEIN A. ALSULTANY AND MOHAMMED S.I. AL BASHA

Abstract

Molluscum contagiosum Virus (MCV) is a type of poxviruses, it cause humans diseases by producing a benign self-limited lesions with multiple umbilicated cutaneous tumors. Aim of this project is to study Epidemiology of MC in Babylon population and Histopathological features of Molluscum contagiosum and Determination the level of tumor necrotizing factor in patients infected with MCV. (This study was conducted in outpatient Clinic of Dermatology of Marjan (Teaching Hospital) as across sectional hospital base study including all patients attending at the period between 29 October 2017 to 30 February 2018. The collection of patients sample was done in dermatology unit by specialist dermatologist who diagnosis the cases and removes lesion from patients by curettage and put it in phosphate buffer saline tube. The demographic information includes age, sex, and address. Samples kept in formalin for histological examination. The frequency of infection according to gender, male 154 (51.25%) and female 146 (48.75%) that mean no statistically differences ingender infection. The results of this work revealed that the distribution of cases according to age groups was (1-10) yrs about (124) 41.33%. While 75 (25%) the average age (11-20) yrs. These result indicated the patients with average age (1-10) yrs. more Susceptible to infected than patient with average age (11-20) yrs. The distribution infection in center of city (Urban) 206 (68.66%) more than periphery of the town (Rural) 94 (31.33%). Distribution of lesion according to body location in face 219 (73%), while less genital area 18 (6%), there was significant difference between MCV infection and site of infection in patients body (P >0.05). (Because the virus-inflamed the cell on the surface of the skin, the as sembly of progeny virions in (a granular matrix inside the cytoplasm forces the cell organelles, inclusive of the nucleus, to the outer edge of the cell. Under the light microscopy those cells stain (as hyaline acidophilic agglomerates, are referred to as molluscum or Henderson–Patters on bodies and are pathognomonic for disease. Maximal magnifications of molluscum bodies) reveal them to be entirely filled (with virions, partial virions and debris. The mean of TNF-α concentration in Molluscum contagiosum (MC) patients sera (36.67) pg/mL, while in control groups (39.18) pg/mL. The study shows no significantly difference between (MC) patients and controls, (p>0.05). Most cases of MCV were detected in urban population than rural population. The face was predominately affected. Most commonly affects preschool and school aged children and the study shows no significant difference between (MC) patients and controls, (p>0.05) according to the tumor necrotizing factor.