Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(602-607)

OCCURRENCE OF QUORUM SENSING GENES AMONG CYTOTOXIC AND INVASIVE MDR PSEUDOMONAS AERUGINOSA

MUNIM RADWAN ALI AND ANFAL MOHAMMED KHUDHAIR

Abstract

Chronic infection, especially in wound and burns units, is an adjunct to infected with P. aeruginosa (20.6%) usually after 7 days after burning or wounded. Sensitivity test for antibiotics represented Imipenem, Norfloxacin and Ciprofloxacin are considered first line therapy of infection caused by MDR P. aeruginosa. The MAR index is a good Risk assessment tool, values for all the isolates were higher than 0.3 reach to 0.9 suggesting the source of these isolates is from highly contaminated environments where there is excessive and randomly use of antimicrobial agents. Cytotoxic isolates were predominant represented of 54 isolates, while only 26 isolates were invasive, as well as 12 isolates considered Cytotoxic and invasive, finally 11 isolates were non cytotoxic nor invasive, so the burn and wound P. aeruginosa isolates almost is cytotoxic or invasive. Most isolates possess one or both QS systems (Las and RhI). Our results indicated that the P. aeruginosa quorum- sensing system lasR predominant in all isolates of P. aeruginosa isolated from wound and burn patients. Spreading bacterial virulence and resistance to antibiotics due to poor medical care (bio-safety and bio-management) and excessive use of antibiotics, creating a new generation of highly virulent bacteria. The study data showed that between the two systems continuity and stability was a system rhl, As it existed in 99-100 isolation of the total 103 isolates tested, while it had three isolates of this system alone, which confirms the real role in the events of the infection. The dendrogram of similarity obtained by ERIC-PCR demonstrated the existence of two main clusters (A-B). This finding can be interpreted in the light of the research data on the apparent role played by gene lasI QS system in the development of antibiotic resistance for its effective role in the development of the bacterial Biofilm, which reduces its ability to antibiotic resistance. The present result confirms the isolates collected were more resemble to be related than unrelated so transmission of P. aeruginosa from patient-to-patient can lead to the spread of clonal strains in other wards of the hospital.

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