Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(598-601)

ESCHERICHIA COLI NISSLE 1917 POTENTIATES TEMEPHOSLARVICIDE ACTIVITY CONFIRMED BY DOWN-REGULATION OF BACTERIAL MONOOXYGENASE GENE EXPRESSION

FADI JAWAD HAMZA AL-SHAMMARI

Abstract

The herein study was designated to investigate the synergistic effect of the non-pathogenic bacterium, Escherichia coli Nissle 1917 (EcN), on temephos larvicide activity against the larvae of Aedes albopictus. According to that, a group 100 larvae were exposed to 0.5 mg/L of temephos and 108cfu of EcN in 100 μL of PBS, TEcN group. Other group of 100 larvae received 0.5 mg/L of temephos only, T group. A control group (C) was provided with the vehicle only. The work for the experiment was ended after a twoday period during which the larvae were monitored for livability. After that, the experiment was repeated for the expression of the gene-related analysis. A quantitative real-time-dependent PCR (RT-PCR) method was done to calculate the expression of alkane monooxygenase gene (alkB). The findings recognized that the larvicide activity in TEcN group was better than that in the T group. When looked at the gene expression (GE), the results were significantly (p<0.05) lower in the TEcN group than that in the T group. The study provides important data about the potentiation effect of Escherichia coli Nissle 1917 on the activity of temephos against the mosquito larvae. This could enhance better use of insecticides in the future.

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