Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(580-586)

ANTIBACTERIAL ACTIVITY AND MORPHOLOGICAL ALTERATIONS IN PATHOGENIC BACTERIA INDUCED BY EXTRACTS OF HIBISCUS SABDARIFFA

MANAL M. ALKHULAIFI, KAHKASHAN PERVEEN NOUF M. AL-ENAZI, NOURA ALDOUSARY, RAGHAD ALZAHRANI AND KHAWLA ALSAMHARY

Abstract

Hibiscus plant having medicinal values proves to contain several bioactive chemicals however; the information pertaining to morphological alterations in bacterial cell due toextract of hibiscus flower are meagre. Therefore, in vitro antibacterial activity of hibiscus flower extracts prepared in Water (HW), ethanol (HE) and methanol (HM) were tested against ten human pathogenic bacteria by well diffusion assay. The HW showed maximum zone of inhibition (30.0 mm) against S. aureus and HM showed least zone of inhibition (8.3 mm) against E. coli. The most sensitive bacteria were S. aureus. MIC value of HW ranges from 0.78 to 6.25 mg/mL whereas for HE ranges from 1.56 to 25mg/mL. The bacterial cells of S. aureus, P. aeruginosa, E. coli and A. baumannii, untreated and treated with the sub-inhibitory concentration of the HW of were observed under Scanning electron microscope for the morphological changes. The SEM analysis clearly showed that the treated cells have several deformities, E. coli cells were rough, corrugated and pores were also observed whereas, A. baumannii treated cells were rough, lumpy and some cells were ruptured, similar deformities were also observed in the cells of S. aureus. Treated cells of P. aeruginosa were thin, deformed and shrunken. The observation shows that hibiscus flower extracts induce morphological changes in bacteria. Thus hibiscus can be a source of natural broad-spectrum antimicrobial agent.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!