Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(561-565)

SCREENING AND IDENTIFICAION OF ACTIVE PHYTOCHEMICALS COMPOUNDS FROM CLITORIA TERNATEA LEAVES

CHRISTINA ISAAC, VALIVITTAN, K. AND P. DHASARATHAN

Abstract

Preliminary phytochemical analysis revealed the presence of secondary metabolites in Clitoria ternatea samples was good. Clitoria ternatea leaves phytochemical screening were performed and recorded in Table 1. Ethanol extracts of Clitoria ternatea (leaves) was found to be different secondary metabolites compared to other solvents. Alkaloids were present in all solvent extracts except in butanol extract. Saponin is present in butanol, ethanol and aqueous extracts and is absent in remaining solvent extracts. Amino acids present in butanol and ethanol extract of Clitoria ternatea and are absent in hexane, chloroform and aqueous extracts. Flavonoids are mostly absent in hexane and chloroform extracts. However, it is found to occur in butanol, ethanol and aqueous extracts of the present study. The study revealed the polarity of the chemical composition of the leaves of Clitoria ternatea. In the present study, the Rf value of compound isolated from Clitoria ternatea by TLC method is given as; three spots from hexane extract (0.13, 0.17, 0.31), five spots from butanol extracts (0.03, 0.06, 0.90, 0.12, 0.15), six spots from ethanol extracts (0.03, 0.08, 0.11, 0.13, 0.17, 0.21), four spots from chloroform extracts (0.13, 0.18, 0.21, 0.33) and three from aqueous extracts (0.80, 0.18, 0.34). The proton NMR spectrum of the compound gave the following compound Octadecanoic acid. It is one unusual base pair present in Clitoria ternatea samples. It is one of the reasons for many of the biological characteristics of the samples. The compound of Clitoria ternatea have been found in vitro to be effective antimicrobial substances against a wide array of microorganisms.

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