Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

PHYSIOLOGICAL STUDY IN GIARDIA LAMBLIA INFECTED MICE AFTER ORAL SUPPLEMENT WITH CLADOPHORA GLOMERATA EXTRACT

SHATHA KHUDHAIER ABBAS, GHAIDAA HUSAIN ABD – ALI AND FIRAS SALIH ABDULHADI

Abstract

This research was conducted to investigate the hematological and hepatic effects in Giardia lamblia infected mice after oral supplement with Cladophora glomerata extract (CGE). The parasite was obtained from the stool samples of the patients who visited AL-Kadimiya teaching hospital after verifying their parasite infection with laboratory samples. Freshwater Cladophora glomerata was collected in October 2017 from bottom of AL-Najaf sea zone. Eighteen white Swiss mice (male and female) each weighing (16–22g) were obtained from the national center for research and drug control with average age between (5-12 weeks). All mice given 1×103 cell of cyst and trophozoite, after (4-8 hours) the stool of al mice were examined and after sure infected by Giardia mice were divided into four groups each group contain 20 mice then inoculated as follow: Group 1: inoculated with 1mL from metronidazole orally by using stomach tube (St) at single dose per/ day. Group 2: inoculated with 1mL of the 128 mg/mL of the CGE extract orally by using (St) at single dose per/ day. Group 3: inoculated with 1mL of the 256 mg/mL of the CGE extract orally by using (St) at single dose per/ day. Group 4: inoculated with 1mL of (PBS) orally by using (St) at single dose per/ day (positive control). Each mouse was anaesthetized with chloroform vapour and pooled blood from mice in each group, was collected by cardiac puncture into heparinized tubes for haematological studies – packed cell volume (PCV), Haemoglobin (Hb), number of leukocytes (WBC), neutrophil, lymphocyte, eosinophil and monocyte. Serum was separated from the blood by centrifuging (3000 rpm) for a period 15 minute. Liver enzyme determinations in serum for GOT and GPT; and ALK. The results of present study show that therapeutic substances caused all effective in the treatment, that best effect was full killed of flaggy to parasite on the sixth day followed by an 256 mg/mL CGE extract and 128 mg/mL CGE extract full killed on the seventh day was compared with control positive. The results also showed a significant increased (P< 0.05) in the mean of W.B.C.,P.C.V. and Hb in all infected group in compare with positive control. On other hand, there was no significant difference (P>0.05) in men of W.B.C., P.C.V and Hb in group 2 and 3 in compare with group 1 (Metronidazole group). The results of Differential white blood cell showed a significant increased (p≤0.05) in the mean of Lymphocyte, Neutrophil, Eosinophil and Monocyte in mice supplement with Metronidazole (500mg/g) and mice supplement with CGE extract (128 mg/mL) compared with mice in control positive and CGE extract (256 mg/mL) groups. The results of biochemical tests showed a significant decreased (p≤0.05) in mean of liver enzymes GPT, GOT and ALK in infected mice compared with positive control group. Our findings lead us to propose that bioactive materials of the C. glomerata was responsible for the anti-giardial activity and have potential for use as therapeutic agents against giardiasis.