ISOLATION AND SCREENING OF HEAVY METAL TOLERANT BACTERIAShruti Handa and Rahul JadhavAbstract Vihar lake and Powai lake located in Mumbai are the two lakes known to supply water to Mumbai. Vihar lake is pristine and situated amidst the hills while the Powai lake has become a victim to pollution through sewage discharges and solid waste dumping on its banks. Approximately twenty bacterial isolates were exposed to lower concentrations (10 ppm, 20 ppm and 30 ppm) of heavy metals (Cu, Cd, Cr, Pb and Ni). Amongst these seven bacterial isolates were found to have considerable capabilities to tolerate the heavy metals. They were further exposed to 50 ppm and 100ppm concentration of the heavy metal salts. Isolate ISL2 was found to tolerate all the heavy metal salts used at 50 ppm but did not show the same tolerance at 100ppm. Isolates ISL3, ISL4, ISL5, ISL6 and ISL7 although did not show tolerance to all the metals but they did show tolerance to few metals at 100 ppm.
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