Ecology, Environment and Conservation Paper

Vol 25, Issue 3 2019; Page No.(1035-1040)

INOCULATING OF PSEUDOMONAS PUTIDA IFO 14796 ON THE EARLY GROWTH OF CORN SEED

Jumadi, Saifuddin Sirajuddin, M.Natsir Djide and Anwar Mallongi

Abstract

Biofortification using plant growth promotion (PGP) bacteria for agronomic intervention is one of the most important strategies to enhance the growth and quality of the maize grain. This study aims to assess the ability of Pseudomonas putida IFO 14796 to stimulate the growth of corn plant. Randomized group design was carried out to assess the effect of Pseudomonas putida IFO 14796 on the early growth of seed corn with three levels of treatment and one of control and 3 replications respectively. The density of P. putida IFO 14796 in the treatment of 3:1 carrier was (5.1x107 CFU g-1), 2: 1 (1.37x107 CFU g-1), and 1: 1 (0.37x107 CFU g-1) and control (uninoculated). The carrier and topsoil were sterilized in an autoclave at 121oC and pressure of 15 pounds for 12 h. Parameters of the root length, number of roots, number of leaves, and plant biomass were collected after 96 h. Data were analyzed by ANOVA and LSD. The effect of the Pseudomonas putida IFO 14796 on the primary root length of the corn seeds was significantly different at p<0.05. The mean of the primary root length of 3:1 (19.53 cm plant-1) was significantly different over the other treatments at p<0.05. P. putida IFO 14796 can stimulate the elongation of the root corn plant so it is potentially developed for improving the quality of the maize grain based on biofortification strategies for chronic diseases prevention. The current work is iron biofortification of corn seed through P. putida IFO 14796 intervention.

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