INFLUENCE OF LIPOPEPTIDE BIOSURFACTANT ‘KANNURIN’ ON PETROCHEMICAL CONSUMPTION EXHIBITED BY BACILLUS CEREUS AK1SHABEER ALI H., PRAJOSH P., SHRUTHI P. NATH AND SREEJITH K.Abstract Bacillus cereus AK1 is a soil isolate previously known to produce a novel antimicrobial cyclic lipopeptide âKannurinâ. This study deals with the influence of Kannurin in diesel consumption exhibited by B.cereus AK1. The primary screening indicated the ability of the bacterium to grow on mineral salt medium by consuming the petroleum hydrocarbon (Diesel) as the sole source of carbon. In continuation, a 7-day batch experiment demonstrated that the bacterium is efficient to utilize 53.94% of the diesel present in the medium under controlled culture conditions. The surfactant property of Kannurin was also studied by oil displacement technique and it showed 28.57 % emulsifying capacity which is also reflected in the enhanced petrochemical consumption exhibited by B.cereus AK1. In this study it is proved that under low concentration or in the absence of Kannurin, B. cereus AK1 is virtually unable to consume diesel fuel. Maximum diesel consumption of 18.24 % was observed when the Kannurin production was suppressed during the biodegradation process. This study reports the direct involvement of Kannurin in the process of diesel consumption exhibited by B.cereus AK1.
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