THE ACTIVITY OF XANTHINE OXIDASE INHIBITOR AND LIPOXYGENASE INHIBITOR OF SEA PLUMES GORGONIAN RUMPHELLA SP. AND HICKSONELLA SP. EXTRACTYunialdi Hapynes Teffu, Ruddy Suwandi and Nurjanah
The coastal communities in Eastern area of Indonesia used the sea plumes as a traditional medicine. Raijuais land is one of the island in East Nusa Tenggara which takes these traditional medicine for relieving arthralgiaor called as rheumatic drugs. The aim of this study was to verify the activity of the sea plumes gorgonian extract as xanthineoxidase and lipoxygenase inhibitor useful as availability of drugs for pharmacy. Xanthineoxidase inhibitors analysis from sea plumes of gorgonian extract with measured the uric acid formed of the inhibition of uric acid formation by xanthine oxidase; and lipoxygenase analysis based on product principle resulted hydro per oxyeicosatetraenoic acid (HPETE). The values of inhibition were measured as the absorbance by UV-Visspectro photometer. The sea plumes of gorgonian extracts was able to inhibit the activity of xanthine oxidase in IC50 of 24.90 Î¼g/mL from bark n-Hexane extract and bark methanol extract with IC50 of 120.83Î¼g/mL; allopurinol IC50 1.03 Î¼g/mL. The result of lipoxygenase inhibitor showed that IC50 value of bark nhexane extract and bark ethyl acetate extract were 2.14 Î¼g/mL and 11.65 ug/mL respectively compared tobaikelain standard IC50 of 0.01ug/mL. The sea plumes gorgonioan extract showed low IC50 value compared to control (baikelain standard). The in vitro result displayed the sea plumes gorgonian extract was less potential as xanthine oxidase and lipoksigenase inhibitor.
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