GCMS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF FRACTIONS OF PIPER BETLE L. VAR. NIGRAJUNAIRIAH, NI’MATUZAHROH, NABILAH ISTIGHFARI ZURAIDASSANAAZ ANDLILIS SULISTYORINI
Black betel (Piper betle L. Var. Nigra) belong to Piperaceae family and one of endemic species of Indonesia. Previous research showed that the antimicrobial activity of methanol extract was better compared to ethyl acetate and n-hexane extracts. This study was aimed to determine antimicrobial activity of fractions of methanol extracts of Piper betle L. Var. Nigra toward pathogenic microbes Candida albicans ATCC 10231, Staphylococcus aureus ATCC 25923, and Escherichia coli ATCC25922. Leaf powder of black betel was macerated using methanol solvents and the methanol extracts was fractionated by vacuum liquid chromatography (VLC). The fractionation process produces 11 fractions. Each fraction was analyzed by GCMS to identify the number and types of bio active compounds. Antifungal and antibacterial activity were determined using diffusion and dilution tests. Data collected from both tests including diameter of inhibition zone (mm) and Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), Minimal Fungicidal Concentration. Data was then analyzed statistically. Result showed that each fraction produces varying amounts and types of compounds. The highest inhibition of E. coli was found infractions of 5, 6 and 11. The highest inhibition of S. aureus was found in fraction 7. The highest inhibition of C. albicans was found in fractions 7 and 11. The MIC and MBC values of the three test microbes were <0.1 mg/mL and 0.1 mg/mL, respectively.
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