Pollution Research Paper

BIO PROSPECTING OF CELLULOLYTIC AND BIO SURFACTANT PRODUCING BACTERIA FOR ORGANIC WASTE TREATMENT

ALMANDO GERALDI, NI’MATUZAHROH, AKEN PUTI WANGUYUN,SUCIPTO HARIYANTO, BRIGITA NUR DIYAN AGUSTIANA, ALIFAH NASTITI,RIZQIA NURUL ALFIYANITA, INDRA GUMILAR, TESALONIKA TETYA VIRGINIA,SITI RIZQIYATUL MUKARROMAH, ANNISA DWI SAVITRI, ERNA ERVIANA NADHIFA,KHAF

Abstract

Increasing amounts of organic wastes such as cellulosic materials and waste oils which are difficult to manage using conventional physico-chemical processes necessiate the need for the development of more effective waste treatment approaches. One of the most attractive efforts is using cellulolyticenzymes and bio surfactants from microorganisms for the treatment of recalcitrant cellulosic materials and waste oils. Those bio molecules are not only effective for specifically removing targeted contaminants, but also easy to produce using cheap raw materials and non-toxic. To obtain native cellulolytic enzymes- and bio surfactants-producing bacteria which are expected to workoptimally for organic waste treatments in Indonesia, we conducted bio prospecting in pine forest and hot spring ecosystems of Raden Soerjo Forest Park, Batu, East Java. As the results, two bacterial strains which exhibit cellulolytic activity and bio surfactant producing ability were isolated and characterized. HS1 strains isolated from hot spring was identified as Bacillus subtilis sub sp. Inaquosorum and is of interest due to its ability to produce cellulolytic enzyme and bio surfactantat high temperature which is a valuable feature for large-scale waste treatments.