Pollution Research Paper

THE ROLE OF CONTINUOUS MODERATE EXERCISE ON HSP70 EXPRESSION AND THE TRANSFORM CELL NUMBER ON ORAL SQUAMOUS CELL MUS MUSCULUS INJECTED BY BENZOPYRENE

ANIS IRMAWATI, SANTIKA RENTIKA HADI, ABDUL HARIS, RETNO PUDJI RAHAJU,THERESIA INDAH BUDHY AND NOOR FAIZAH BALQIS

Abstract

Non-communicable diseases are currently responsible for most global deaths,and cancer is estimated to get first rank as a cause of death. The prevalency of cancer is 1.4% out of 1000 people.Cancer is mainly caused by two factors: genetic and environmental, one of them being is acarcinogenic agent benzopyrene that found in cigarettes. Physical exercise can decrease blood glucose and fatty acid levels within the blood but its effect on cancer prevention, especially oncaspase-3 expression, is still unknown. To prove the role of continuous moderate exercise on Hsp70 expression and the number of transform cell on oral squamous cell Musmusculus injected bybenzopyrene. 18 mice were divided into three study groups : control group 1 (K1) not given any physical exercise and benzopyrene, control group 2 (K2) not given any physical exercise but induced with 0.08 mg benzopyrene, treatment group (P) were given physical exercise in moderate intensity and induced with 0.08 mg benzopyrene. Buccal mucosa tissue samples were taken and stained for immunohisto chemistry and examined under a light microscope at 400x at 10 different angles. There was no difference among the three groups on Hsp 70 expression (p = 0.874), but there was significant differences among the three groups on the number of transform cell (p = 0.000).The conclusion of this study was continuous moderate exercise have no effect to Hsp70 expression, but could decrease the number of transform cell on oral squamous cell Musmusculus.