NEW HAPLOTYPES OF BLACK-BEARDED TOMB BAT(TAPHOZOU SMELANOPOGON) FROM PUNCAKWANGI CAVE(EAST JAVA, INDONESIA)Muhammad HilmanFu’adil Amin, Nabilatun Nisa’ and BambangIrawan
DNA barcoding is a new method for identifying species from any biological species using standardized target gen and/or intergenic target. However, identification success is only as good as the underlying database. In this study, cytochrome c oxidase I (COI) sequences of 6 individuals were generated and analyzed from Taphozou smelanopogon. Genetic distance, Neighbor-Joining (NJ) tree, and haplotype network analyses were performed to identify new haplo type. COI sequence analysis from the present study, combined with downloaded sequences from NCBI GenBank, revealed two new COI haplotypes from East Java, Indonesia.Population of T. melanopogon in East Java have unique haplotypes which are discrete from the haplotypesfrom Genbank (Myanmar/Vietnam) with pairwise distances 0.27-0.28 and number of segregating sites among samples are 19 nucleotides and number of parsimony-informative sites are 17 nucleotides,demonstrating that population in this study are highly isolated. The existence of isolated haplo types highlights the significance of considering genetic diversity in developing sustainable management and conservation plans for biodiversity.
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