DNA BARCODING OF INVASIVE FRESHWATER FISH REVEALS TWO SPECIES OF AMPHILOPHUS FROM TWO DAMS IN BRANTAS STREAM, EAST JAVA, INDONESIAMuhammad Hilman Fu’adil Amin, Alfiah Hayati, Win Darmanto and Manikya PramudyaAbstract DNA barcode is a new DNA based identification method using cytochrome c oxidase I (COI) fragment as standard for identifying species in animal kingdom. In this study, we applied DNA barcode to identify invasive species in genus Amphilophus(Mayan Cichlid) from two dams in Brantas watershed, East Java,Indonesia. 16 samples of Amphilophus were collected from Karangkates Dam and Lahor Dam with various body color. Total genome was extracted from muscle tissue, and amplified using universal primer for COI,and sequenced. COI fragments were identified using BOLD Identification System and unrooted Neighbor-Joining (NJ) tree, pairwise Kimura 2-parameter (K2P) distance and haplotype network were performed. COI sequence analysis from this study showed that there are two species in both dams, A. citrinellus and A. trimaculatus. Pairwise distances between two species was 4.8% and number of segregating sites among samples are 31 nucleotides, demonstrating that the population consist of two species. Haplotype-bodycolor mapping showed that A. citrinellus has more color polymorphism, from dark to bright color, compared to A. trimaculatus which have dark color. From this finding, DNA barcode is a significant method for the species identification and produce a functional framework for exploring species invasion.
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