Ecology, Environment and Conservation Paper

PLANT GROWTH PROMOTION BY VOLATILE ORGANIC COMPOUNDS PRODUCED BY CHRYSEOBACTERIUM RHIZOPLANAE ISOLATED FROM VIGNA RADIATA

O.N. Shemshura, Zh.N. Shemsheyeva, A.K. Sadanov, M.B. Alimzhanova, S.T. Daugaliyeva, G.A. Mombekova and Zh.K. Rakhmetova

Abstract

A bacterial isolate was isolated from the mung bean (Vigna radiata) root nodules, cultivated in the Turkestan region of Kazakhstan. It was morphologically and biochemically identified as Chryseobacterium rhizoplanae and this identification was confirmed by 16S rDNA sequencing. It has been established that C. rhizoplanae stimulates linear growth of the root and stalk of the mung bean: it is higher, as compared to the control, by 53.6% and 32%, respectively. Compositional analysis of the C. rhizoplanae culture broth revealed volatile compounds of acetoin and 2,3-butanediol, which are one of the factors causing the growth of mung bean. Presowing treatment of mung bean seeds with acetoin and 2,3-butanediol at a concentration of (250 mg/L) stimulated root growth to be higher (as compared to the control) by 21.4%-19.2%, and stem growth – by 14.5%-22.3%, respectively. The results indicate a great potential of C. rhizoplanae 1M strain as the basis for the creation of a biological product to stimulate the growth of Vigna radiata, one of the possible mechanisms of which is the production of acetoin and 2,3-butanediol.