ENHANCED ARID AREAS ON-SITE ARYL-DI-ALKYLPHOSPHATASE BIOSENSOR BUILT ON AU-NANOPARTICLES DECORATED GRAPHENE HYBRID NANO-SHEETS FOR DIAZINON DETECTIONMuntasir Ali Al-Najjari, Othman Mousa Hakami, Abdul Jabbar Al-Rajab, Emad Mohamed Ebada and Taharh Zelai
In the recent years, environmental pollution has become a major global concern due to the adverse human health effects. Some of the major sources that contribute toward this environment contamination are the massive use of organophosphorus pesticides in agriculture and public health pest control. These toxic agents are affecting water resources, air and soils. Therefore, rapid, sensitive, selective and reliable detection of these toxic compounds is crucial. For this purpose, many biosensors had been manufactured based on various emergent nano-structured materials. Despite their acceptable sensitivity level and detection limits, these nano-assembled biosensors are tested under controlled conditions of temperature room. Therefore, they might be not accurate for on-line detection of pesticides in arid areas at high temperature ranges.This paper aims to design, build and operate a hybrid bio nano-assembly sensor for the on-site detection of the organophosphorus insecticide diazinon (as model). The sensing activity of this biosensor has been performed by the use of an on-site E. Coliâs hydrolase enzyme self-assembled on gold nanoparticles decorated grapheme oxide. To the best of knowledge, this is the first study of its kind on detection of OP pesticide (diazinon) by bio-sensor contains enzyme from the local environment of Jazan in Saudi Arabia.
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