Pollution Research Paper

Vol 38, Issue 2, 2019; Page No.(447-451)

BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF CADMIUM RESISTANT BACTERIAL STRAINS

SNEH LATA, HEMANT PREET KAUR AND TULIKA MISHRA

Abstract

The present study was done to investigate the morphological, biochemical as well as molecular characterization of isolated strains for cadmium bioremediation. The Microbial strains were isolated from the soil and effluent samples of electroplating, battery as well as steel industry by using nutrient agar media. Those strains were checked for their resistance to cadmium. The maximum cadmium concentration (1000-1500ppm) tolerating strains were chosen and furthermore they were characterized morphologically by studying Colony Morphology and Gram's staining,then biochemical characterization was performed by using several biochemical tests described below. The strains were further subjected to molecular characterization through 16SrRNA sequencing method and analyzed by BLAST and phylogenetic studies. The strains isolated were Gram positive and aerobic in nature. Both have shown different response for biochemical tests.BLAST analysis of 16SrRNa sequences showed the relevance of strain E Cd004 with Bacilluscereus CCM 2010 strain (95% identity) and S-Cd005 with Cuprividus plantarum ASC 64 (98%similarity) and phylogenetic analysis showed the evolutionary distances among them.

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