Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 2, 2019; Page No.(347-354)

ISOLATION AND CHARACTERIZATION OF LOCAL ISOLATE BACTERIA IN REDUCING MOLYBDENUM FROM MALAYSIA SOIL

NURLIZAH ABU BAKAR, MOHD YUNUS SHUKOR AND AHMAD RAZI OTHMAN

Abstract

Molybdenum-reducing bacteria were isolated from soil and water samples throughout Malaysia and one isolate from workshop area in Seri Kembangan, Selangor was identified to reduce molybdenum to molybdenum blue in less than 48 hours with the most intense blue-colour colony formation. Further analysis using 16s rDNA ribosomal gene sequence comparison and molecular phylogenetics analysis showed that isolate Point 1 is Gram-positive coccus and grouped under genus Staphylococcus and was submitted to Genbank under accession number of EU828636 as Staphylococcus sp. isolate liz1. The characterization studies showed that the bacterium gave the highest Mo-reducing activity under the combination of 1% glucose, 50 mM molybdate, 5 mM phosphate, at pH 6.0 and temperature 37 °C in 48 hours of incubation time.

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