OXIDATIVE STRESS RESPONSES IN GILLS OF TILAPIA (OREOCHROMIS NILOTICUS LINNAEUS, 1758) TO CADMIUM EXPOSUREKiki Syaputri Handayani and Agoes Soegianto
Heavy metal accumulation in fish depends on many factors including several chemical interactions, their concentration in water and exposure time with heavy metals. One of heavy metals is Cadmium (Cd) which is dangerous for fish body. Tilapia as euryhaline species is a good research model for osmoregulation of fish. Oxidative stress biomarkers were the level of catalase (CAT), reduced Glutathione (GSH) and malondialdehyde (MDA) as a lipid peroxidation marker. The objective of this research was to evaluate the effects of sublethal Cd concentration on oxidative stress in gills of tilapia that were given Cd concentration 2.5 ppm. Research was conducted at three assay concentrations on fish gills, the concentration of CAT, GSH and MDA with ELISA assay. The levels of CAT in gills were significantly lower at 2.5 ppm than control. The levels of GSH in gills were significantly higher at 2.5 ppm than control. The level of MDA in gills was higher at 2.5 ppm than control. In conclusion, East Java strain tilapia (O. niloticus) demonstrates there is redox balance disturbance at treatment group exposed by Cd. The existence of Cd causes decreasing of CAT level and increasing of MDA level.
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