A STUDY ON THE WANING EFFECT OF OIL SPILL ISOLATED MIOCROALGA COELASTRELLA SP. ON A SYNTHETIC DYE, ERIOCHROME BLACK TK.P. DEEPA, A. PANNEERSELVAM AND N. THAJUDDIN
The major application of dyes are textile industries and the effluents from factories will retain in the ecosystem. Industrial residues with non degradable hydrocarbons cause long term ill effects. In this study, a microalga, Coelastrella sp. NTAPD 01 isolated from oil polluted area has been used for degrading an azo dye, Eriochrome black T with batch culture system. The conditions for maximum degradation of dye had been optimized as 30 Â°C, 10mg/L dye concentration, pH of 8.0 with 10% inoculum. At this condition, 85% degradation of dye occurred in 20 days. The decolorization of dye was confirmed by UV Spectrophotometry and the chemical breakdown was analyzed using FT-IR, GC-MS, HPLC analysis. The toxicity of algae treated dye on plants was studied by germination pattern of dicot and monocot seeds. The germination rate, radicle, plumule and leaf length were studied comparing with the seeds germinated in distilled water. The dye degraded algae was studied under confocal laser scanning microscopy using Nile red stain for lipid accumulation. This study resulted in the identification of promising alga Coelastrella sp. NTAPD 01 efficiently degrading Eriochrome Black T via non-hazardous way with non-toxic by-products.
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