Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 1, 2019; Page No.(139-145)

EFFECT OF PSIDIUM GUAJAVA LEAF EXTRACT AGAINST SHRIMP PATHOGENS AND ITS ANTICANCER ACTIVITY ON MCF-7 CELL LINE

AVITTA OLIVERO, D.R. DARJILY, S. PRIYA AND R. SHANMUGAPRIYA

Abstract

The shrimp samples selected for the study was collected from Ernakulam district, Kerala. From the infected shrimp, bacterial pathogens were isolated by the standard isolation techniques used for the isolation and identification of sea food pathogens. The major group of bacteria which infected shrimp includes Vibrio cholerae, Salmonella sp. E.coli and Staphylococcus aureus. The present study is focused on the isolation and identification of bacterial pathogens causing spoilage in shrimp varieties. Tiger shrimp and white leg shrimp were selected for the study as these are highly consumed and widely available. The plant samples of Psidium guajava, Avicennia marina and Cinnamon cassia were collected from Ernakulam district and antibiotic sensitivity for various bacterial strains was tested. The ethanol and acetone extracts of Psidium guajava, Avicennia marina and Cinnamon cassia showed antibacterial activity against Vibrio cholerae, Salmonella sp. E.coli and Staphylococcus aureus. The leaf extract of Psidium guajava and Avicennia marina were tested against MCF-7 cell lines for their combinatorial anti-cancer activity. Phytochemical constituents were analyzed for Psidium guajava since it exhibited higher antibacterial activity and also it showed the presence of saponins, flavanoids, phenols, moderate presence of steroids and glycosides. FTIR analysis of the Psidium guajava was studied in order to find the active functional group in the plant extract.

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