Ecology, Environment and Conservation Paper

Vol 25, Issue 1 2019; Page No.(450-455)

ISOLATION AND IDENTIFICATION OF BACILLUS SPP. THERMOPHILIC OBLIGATE PRODUCING SERINE ALKALINE PROTEASE FROM HOT SPRING IN SUNGAI ABU, KERINCI, JAMBI, INDONESIA

Arzita, Syamsuardi, Anthoni Agustien and Yetria Rilda

Abstract

This study aimed to isolate Bacillus spp. thermophilic obligate generating serine alkaline protease in a hot spring in Sungai Abu, Kerinci, Jambi, Indonesia. The research was conducted in several stages, namely; sampling, isolation, screening, and testing of protease activity. Determination of species was done with morphological, microscopic, biochemical, and molecular identification. Of the 28 isolates, all produced proteases in the medium of skim milk agar (SMA) pH 8.0, 60 oC, but only two isolates that had high potential to produce proteases in basal media with an activity of 0.134 and 0.186 U/mL and index proteolytic of 2.68 and 4.63, respectively. The results of the molecular identification of 16S r of RNA on both isolates show that they were identified as Bacillus sonorensis and Bacillus aerius.

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