LOW TEMPERATURE PRESERVATION OF CYANOPHYTA (GENUS SPIRULINA)Daria I. Petrukhina
New results of cryopreservation of cyanoprokaryota genus Spirulina, Spirulina laxissima, have been obtained. In the current study, the strain SAG 256.80 of filamentous cyanoprokaryota S. Laxissima (previous versicolor) under freezing temperature minus 80 Â°C with cooling rate of 1 Â°C/min have been preserved. Optimal cryoprotectants and this concentration have been investigated through S. laxissima cell viability after thawing using flow cytometry. Solutions of glucose and dimethyl sulfoxide (DMSO) have been efficient cryoprotectants, leading to the successful preservation of six month at temperature down to minus 80 Â°C of S. laxissima. In general application of glucose solution gave better recovery rates and optimal concentration solutions of glucose and DMSO have been determined as 10 %. Furthermore, has been determined the growth characteristic of S. laxissima as maximal growth rate after six months storage at minus 80 Â°C. Since cyanoprokaryota S. laxissima strain SAG 256.80 not form the centrifuged cell pellet, the effect of exposure time with DMSO on survival of S. laxissima has been evaluated.
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