Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

DETECTION OF MUTATIONAL P110-AND P57 GENES EXPRESSION IN RELATION WITH RETROVIRAL INFECTIOUS TISSUES FROM IRAQI FEMALE WITH BREAST TUMORS

SHAKIR H. MOHAMMED AL-ALWANY, ISRA’A MAHDI AL-SSUDANI, A.A. AMBER H ABDUL AMEER, SAAD HASAN MOHAMMED ALI, SURA DHAFER DAWOOD ALAZIZ AND AHMED A. ABED-ALZUWAID

Abstract

A total number of (120) formalin-fixed, paraffin embedded breast tissues. The mean of age of the patients with breast carcinoma was higher (41.75±8.763 years) than the mean age of the benign tumors group (39.47 ±7.825 years) and the mean age of those females in the group of healthy control (37.33± 7.556 years). The poorly differentiated grade (III) of breast cancer (BC) constituted 42.5% (17/40) whereas those with moderately (II) and well (I) differentiated grades BC constituted 30% (12/40) and 27.5% (11/40), respectively. Detection of Human Mammary Tumor Virus (HMTV) envelope gene-CISH reaction in tissues with BC was observed in 16 out of 40 (40%). While HMTV envelope gene-CISH in the tissues from benign breast tumors was detected in 17.5% (7 /40). No positive – for HMTV envelope gene-CISH reaction was detected in apparently healthy breast tissues of the control group. The positive P110- IHC reactions were detected in 45 % (18 out of 40 cases) of malignant breast tumor. While, in benign breast tumor was expressed in 37.5%. No signal was reported in the tissues of control group. The positive of mutated p57-CISH reactions were disclosed in 52.5% (21 / 40) of malignant breast tumor cases. Whereas, in benign breast tumor the positive results was 47.5% (19 / 40) of mutated p57-CISH -gene expression. No signal was reported in the tissues of control group. Our results indicate that the HMTV might contribute to the development of subset of breast tumors. The present results of the rates of defects or mutations in the P110-; and P57 genes in relation to the grade of breast cancer tissues also could point for their occurrence and contribution as early events in breast carcinogenesis.