MOLECULAR DETECTION AND PHYLOGENETIC TREE ANALYSIS OF SALMONELLA PULLORUM ISOLATED FROM BROILER CHICKEN IN AL-DIWANYIA PROVINCE, IRAQHIBA TURKEY ATYIA AND ABBAS HADIJASIM AL-MAHMOUDIAbstract The current molecular-based analytical study was initiated to detect the infection occurrence of Salmonella pullorum in broiler chicken plus to understand some of the evolutional features of the study isolates and compare them with global isolates. Here, 25 cloaca fecal samples were collected from 25 broiler chickens of a breeding hall in Al-Diwaniyah City, Iraq. The samples were subjected, first, to polymerase chain reaction (PCR)-based exploring for the presence of this bacterium. Then, some of the positive samples from the PCR were sent out for partial sequencing. Both PCR and sequencing employed 16S rRNA gene as a target. The sequencing results were studied for evolution using phylogenetic analyses that were ended up with building a phylogenetic tree to compare our isolates with some global isolates of this bacterium. The PCR method showed the occurrence of S.Pullorum in 8 (32%) out 25 samples. The sequencing, for four-PCRpositive samples, confirmed the presence of this bacterium in the positive samples. When studied the phylogeny, the confirmed isolates (No.1 (MH752058), No.3 (MH752060), and No.4 (MH752061)) showed 99% similarity to the NCBI isolate, MH421997.1, an isolate from Colombia. Moreover, the current study isolate No.2, MH752059, revealed 99% matching to the NCBI isolate, MH446374.1, another isolate from Colombia. The current molecular and phylogenetic study reveals the actual present of S.Pullorum in the broiler chickens of the studied city plus shows evolutional characteristics and links to some global isolates.
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