Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 4, 2018; Page No.(1273-1277)

A COMPARATIVE STUDY ON TOTAL PROTEIN, TOTAL CARBOHYDRATE CONTENT AND ANTIOXIDANT ACTIVITY OF AEGLE MARMELOS PLANT PARTS BY FRAP ASSAY

NIDHI SHARMA AND WIDHI DUBEY

Abstract

Aegle marmelos plant commonly known as Bilva belongs to family of aroma ‘Rutaceae’ and thrives well in a wide range of climatic conditions. This plant can be seen almost everywhere and mostly near the temples due to its mythological importance. Charak, the “father of medicine” from ancient Indian history also mentioned the importance of Aegle marmelos in medicine in his treatise. This study was aimed to evaluate and compare the total content of primary metabolites in Aegle marmelos root, stem, leaves, bark, fruit peel, and pulp and to check and compare their Antioxidant activity. Total carbohydrate content was estimated using anthrone reagent and protein content was calculated using Lowry’s method. Ferric reducing antioxidant power (FRAP) assay was used to test the antioxidant activity. Total carbohydrate content was found to be highest in fruit part and lowest in bark, ranging from 0.26±0.03 to 126.49±1.62 mg/g dry wt. of sample and protein content ranged from minimum of 35.3±1.3 mg/g dry weight of sample in the bark to maximum of 182.91±1.97 mg/g dry wt. of sample in the plant leaves. FRAP assay revealed that bark of the plant shows relatively low antioxidant activity compared to other parts, which ranged from 53.66±0.53μg/mL to 285.88±0.48 μg/mL of sample (Fe II eq.). This study signifies the nutraceutical and antioxidant potential of A. marmelos plant which contributes to its medicinal property. As Rajasthan covers 60% area of the Thar Desert, growing this plant under afforestation program would prevent the growth of desert with fewer efforts and it would also serve as a potential source of medicine under AYUSH BHARAT program of Government of India.

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