Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 4, 2018; Page No.(1195-1199)

OPTIMIZATION OF KERATINASE PRODUCTION BY DERMATOPHYTES USING POULTRY FEATHERS IN-VITRO

ELANGOVAN ELAVARASHI, ANUPMA JYOTI KINDO AND PERUMAL KUMAR

Abstract

The poultry feathers constitute about 90% protein, which is found abundant as a poultry waste worldwide. The accumulation of the feathers results in keratin wastage and environmental contamination. This study is aimed to biodegrade the poultry feathers and determine the keratinase activity of dermatophytes by spectrophotometry and estimation of amino acids by High Performance Liquid Chromatography (HPLC) and optimize Polymerase Chain Reaction (PCR) in detection of the keratinolytic protein of dermatophytes using newly designed specific primers. The dermatophytes were inoculated in Soyabean Casein Digest Broth (SCDB) enriched with poultry feathers were utilized for the Keratinase production. The keratinase was produced by all the dermatophyte strains, especially T. interdigitale showed abundant keratinase production of 31 units and essentials amino acids like histidine, leucine, valine and methionine were produced after biodegradation at concentrations of 120, 112, 89, 75 μmols respectively per 100 mL broth. PCR targeting the keratinolytic protein was observed from all dermatophytes, which resulted in 300 bp amplified product. The enzymatic poultry feather degradation results in bioconversion of the keratin waste from contaminant to an animal feed supplement. Therefore, the microbial keratinase from dermatophytes act as a biocatalyst in poultry waste management and can be utilized for de-hairing process in the leather industry.

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