MONITORING AND STUDYING THE MICROBIAL BIODIVERSITY AND ITS EXOPOLYSACCHARIDES PRODUCTION OF SOME NATURAL ENVIRONMENTS OF TABUK REGION (TABUK), KSAMADEHA O.I. GHOBASHY AND MOHAMED M.I. HELAL
Soil microbiota is the main limiting factor of the soil nutrients and efficiency for planting and productivity. However it is important to survey and identify the wild microbial communities especially in the non-studied areas. In our research we studied soil samples from different areas of Tabuk region to isolate a new bacterial isolates to examine its exopolysaccharide (EPS) productivity. Out of 210 soil bacterial isolates were isolated from 165 soil samples derived from 11 different places in Haqel area, Tabuk region and identified by 16 S rRNA PCR technique. It is found that Bacillus sp. act as about 70.4% of the total isolates of soil microbiota. The bacterial isolates were examined for its EPS productivity and choose the highly significant EPS producer isolate and examined for its optimized production condition to gain the highest productivity with low cost. According to the identification by 16S rRNA PCR sequence technique, Bacillus licheniformis SML_M193 is the most EPS producer and it will undergo more experiments for optimization of the production conditions which include both environmental and nutritional factors. Our results revealed that using the modified fermentation medium will increase the EPS productivity from 3.89 g/L to 4.80 g/L when the producer isolate incubate aerobically at initial pH 7.0, temperature 37 oC and all control medium constituents (casein hydrolysate and the salts) except using fructose 3% instead of glucose 3% for 48hr.
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