Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

CELLULAR VIABILITY STUDY OF ASCORBIC ACID SUPPLEMENTATION IN RABBIT BONE MARROW MESENCHYMAL STEM CELL CULTURE (IN VITRO STUDY)

RISKA DIANA1, ANDRA RIZQIAWAN2*, OKKY PRASETIO1, PURWATI3 AND COEN PRAMONO

Abstract

Growth factor and co-factor have an important role in the process of differentiation and proliferation of mesenchymal stem cells into osteoblast cells due to their osteoinductive properties. Ascorbic acid is a cofactor supplement to mesenchymal stem cell culture media that can affect cell viability at the proliferative stage, induce osteoblast differentiation, and increase the formation of collagen fibers. However, optimum dose of ascorbic acid supplementation to bone marrow mesenchymal stem cell culture media has not been established. This study examined the optimal dose of ascorbic acid supplementation in rabbit bone marrow mesenchymal stem cell culture media. The samples were divided into 7 experimental groups (with ascorbic acid supplementation) and 1 control group (consisted of basic medium only). The doses of ascorbic acid supplementation in experimental groups were 5 g/mL, 50 g/mL, 100g/mL, 200 g/mL, 250 g/mL, 300 g/mL, and 350 g/mL. The amount of cell viability was determined using MTT assay and analyzed with Elisa reader. The result of this study showed that rabbit BM-MSC supplemented with 200 g/mL ascorbic acid exhibited the highest number of viable cells (117%) compared to the other groups (p<0.05). The optimal dose of ascorbic acid supplementation in rabbit BM-MSC culture media is between 50 g/mL and 200 g/mL.