BIOREMEDIATIN OF PETROLEUM CONTAMINATED SOIL USING OYSTER MUSHROOM LOG WASTE (OMLW), AZOTOBACTER VINELANDII AND A PETROPHYLIC CONSORTIUMPUJAWATI SURYATMANA, ADHYASA MUDA ZANNATAN, ANISA ROSALINA SYLVIA, MIEKE ROCHIMI SETIAWATI, SYAFRIZAL, ZULKIFLIANI, ASRI PENY WULANDARI7 AND ARGA RIZTAMA
Three bioremediation strategies to enhance biodegradation performance of petroleum contaminated soil are investigated and compared: (1) Soil Amendment using Oyster Mushroom (Pleurotus pulmonalis) logbag waste (OMLW), (2) Bioaugmentation using a petrophylic consortium consisting of Pseudomonas sp., Actinomycetes sp., and Aspergillus sp., and (3) Bioaugmentation using the bacteria Azotobacter vinelandii. Randomized block design (RBD) with four treatments (three treatments + control) and three replications were used. Soil samples from University of Padjajaran were contaminated with 5% petrolleum oil. Total Petropleum Hydrocarbon, Gas Chromatogram Analysis, and Total Plate Count were carried out to investigate the bioremediation potential of each treatment. Results showed that Petrophylic Consortium and OMLW had nearly identical results and are among the highest of the three, at 83.97% (0.036183 day-1) and 81.67% (0.033177 day-1) of TPH removal respectively. Azotobacter vinelandii however lagged behind and only achieved 60.29% with a rate of 0.01652 day-1. All treatments demonstrated the ability to degrade aliphatic hydrocarbons by converting the long chain compounds into short chain petroleum hydrocarbons, and cleaving aromatic polycyclic ring into different intermediate products. In Conclusion, OMLW showed tremendous potential as an alternative for biostimulation uses. it is easily available as an agricultural waste and even easier to use for bioremediation, requiring little maintenance, and can be used as is. The mixing of biodegrading bacterial and fungi in a mixed consortium also showed compelling biodegradation capabilities similar to other well-known bioremediation methods. And we confirmed Azotobacter vinelandii as a valuable bacterium for bioremediation use. The ability to enhance the performance of biodegrading bacteria by producing biosurfactants and essential N-nutrients for bioremediation could prove to be of tremendous value when mixed with other petrophylic microbes in a mixed consortium.
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