ESTIMATION OF PROXIMATE COMPOSITION AND ISOLATION OF BIOGENIC AMINE FORMING BACTERIA IN MARINE EDIBLE FISH, SARDINELLA LONGICEPS AND STOLEPHORUS INDICUS COLLECTED FROM MARKET AND LANDING CENTERA. JULIYANA LEBENEA AND G. CHITRAAbstract Seafood may harbour a number of biological, chemical and physical hazards, the most prevalent of which are biogenic amines (BAs) and pathogenic bacteria. Biogenic amines are low molecular weight organic bases with biological activity that are formed in foods by microbial decarboxylation of the corresponding amino acids. Seafood is susceptible to contamination by biogenic amine producing microorganisms at different points of the food chain. The aim of this current study was to compare the proximate composition and microbiological spoilage in marine fish species such as Sardinella longiceps and Stolephorus indicus samples were collected from Ukkadam, Lorryphet fish market, Coimbatore, Tamil Nadu and fresh fishes were collected from landing center in Tuticorin, Tamil Nadu. The proximate compositions such as protein, lipid, carbohydrate and moisture contents were recorded and reduced proximate composition of the fish samples were observed in market fishes. The total viable count (TVC) in edible part of sardine, Sardinella longiceps and anchovy, Stolephorus indicus samples procured from the market fishes revealed that the uncountable bacterial load on nutrient agar plates. Different pathogenic bacterias were isolated from the samples collected from the local market and the isolated bacterias were Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Proteus vulgaris and Pseudomonas aeruginosa. The isolated bacteria are biogenic amine producing bacteria and the edible fish become toxic due to the biogenic amine formation especially histamine.
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