Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 3, 2018; Page No.(738-743)

BOX-PCR FINGERPRINTING FOR MOLECULAR GENOTYPING OF PSEUDOMONAS AERUGINOSA

RANA M. ABDULLAH AL-SHWAIKH, ARWA M. ABDULLAH AL-SHUWAIKH AND ABBAS FALIH AL-ARNAWTEE

Abstract

In this study one hundred isolates of Pseudomonas aeruginosa have been collected from Iraqi patients with different complains from different sources during the period from September to December, 2014. After bacteria isolation and identification, Pseudomonas aeruginosa was confirmed in 75 isolates. The BOX – PCR method was used to detect the relationship among these Pseudomonas aeruginosa isolates, the fingerprinting patterns of the isolates were shown 14 bands on gel electrophoresis, with molecular weight ranging between (140-1000 bp) among the 75 Pseudomonas aeruginosa isolates with fragment 140 bp, 180 bp, 200 bp, 250 bp, 300 bp, 350 bp, 400 bp, 450 bp, 500 bp, 550 bp, 600 bp, 650 bp, 780 bp and 1000 bp, found in 13.33%, 20%, 37.33%, 44%, 65.33%, 26.66%, 46.66%, 41.33%, 37.33%, 48%, 34.66 %, 26.66%, 35.33%, 33.33%, respectively. Genetic connections of different isolates of Pseudomonas aeruginosa was done by using BOX - PCR method and Dendogram analysis of the results showed the genetic relationship between 12 Clones of Pseudomonas aeruginosa isolates, while 17 isolates contained different genotypes and 4 untypeable isolates. In conclusion the BOX method is reproducible, easy, fast and cost effectiveness method to study the relatedness between different bacterial isolates.

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