Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 2, 2012; Page No.(281-290)

STUDIES ON CHARACTERIZATION OF EXTRACELLULAR ALKALINE PROTEASE FROM A MUTANT ASPERGILLUS FLAVUS AS2

M. ROJA RANI AND N.N.PRASAD

Abstract

After our previous purificational studies on Alkaline protease from a mutant of, Aspergillus flavus AS2 Characterisation of the purified enzyme was done from the culture supernatant by employing various parameters. The optimum pH and temperature for the activity of alkaline protease was previously found to be 8.5 and 550C and stable in the pH range 7.0- 8.5. The thermo stability exhibited by protease ranges from 30-500C.Among various protease inhibitors PMSF strongly inhibited the enzyme activity revealing that the enzyme in the present study is serine alkaline protease. Ca2+ enhancing effect on the activity of the enzyme. High level of hydrolytic activity was shown by casein and also found that purified alkaline protease digested the human blood clot, coagulated egg white to soluble form and also dehairing the goat skin after prolonged incubation. The protease showed good compatibility and stability in the presence of CaCl2 and glycine with detergents. The enzyme retained 20-40% activity with most of the detergents tested even after 3hrs. The supplementation of the enzyme preparation in detergents completely removed the blood stain of the cloth. The enzyme followed a typical Michaelis-Menten kinetics and the apparent Km value was found to be 3.2mgmL-1.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!