EVALUATION OF PGPR TRAITS OF SOME PHOSPHATE SOLUBILIZING MICROORGANISMS ASSOCIATED WITH FOUR AGROFORESTRY TREE SPECIESN. KISHORE, M. RAMESH AND S. RAM REDDYAbstract A study on distribution of phosphate solubilizing bacteria and fungi revealed that they are distributed more within the rhizosphere soil at the rate of 104 (PSB) and 103 (PSF) CFUg 1 soil. Phosphate solubilizing abilities were initially screened through agar plate method and later confirmed in broth medium. Efficient isolates were selected and analyzed for additional PGPR traits. Different isolates were found to be positive for indole acetic acid, gibberellic acid, siderophore, glucanase, HCN and ammonia production. Isolates with efficient P solubilization ability along with more number of PGPR traits were selected and further evaluated for their ability to sustain these characters under different carbon and nitrogen sources. Further their ability to solubilize the inorganic phosphates with increasing salt stress was evaluated. Tolerance to heavy metals and antibiotics was also studied. Two promising isolates of bacteria (PSB-8, PSB-9) and three fungal isolates (PSF-8, PSF-11, PSF-12) were selected for further studies. Bacteria were observed to be more efficient in seed germination and root colonization ability with regard to all four selected plant species. The two bacterial isolates were tentatively identified as Micrococcus luteus (PSB-8) and Pseudomonas aeruginosa (PSB-9). The two fungal isolates were identified as Aspergillus awamori and Penicillium digitatum.
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