Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 2, 2018; Page No.(474-481)

ISOLATION OF A SALT- AND METAL-RESISTANT METHYLOTROPHIC BACTERIA STRAIN ASSOCIATED WITH RETAMA MONO SPERMA (L.) BOISS

N. SELAMI, K. HACHEM1, C. AIBECHE AND M. KAID-HARCHE

Abstract

A pink-pigmented facultative methylotrophic bacteria (PPFMB) was isolated from Retama monosperma root nodules. Due to their capacity to promote plant growth and to reduce environmental contamination, the bacteria were characterized by detailed 16S rRNA gene analysis, heavy metal tolerance, and visually determining the colonization behavior of the methylotrophic strain on and inside the roots of Retama through light and electron microscopy. Microscopy revealed that bacterial cells can colonize the root hair zone, the root elongation zone, and at lateral root emergence sites. However, the bacterial cells were also found in the intercellular spaces inside the root, especially in the epidermal cell layer and underneath this layer, forming sub-colonies inside the host cells. The heavy metal resistance analysis showed that this isolate was highly tolerant to salinity, Zn, and Cd up to 800 mM, 20 mM, and 16 mM respectively, and was less tolerant to Ni and Cu, with viability declining at eight mM.

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