ISOLATION, ANTIBIOTIC PROFILE AND GLYPHOSATE UTILIZATION OF PSEUDOMONAS AERUGINOSA FROM RICEPADDY SOILS IN EBONYI STATE, NIGERIAGODWIN NKWUDA NWONUMARA, CHIBUIKE IROEGBU, BONIFACE NWOFOKE UKWAH, MOSES IKECHUKWU BENJAMIN, JOHN IDENYI NWOKPURU, OGONNA CHRISTIANA ANI, AMOS NWORIE, AUGUSTINE OKOH OKPANI, OBI IHUOMA, EGWUOM GODSWILL BLESSING , VICTOR UDOH USANG AND NSE OKON UMONG
The roles of micro-organism in human diseases and in environmental restoration are enormous. This laid the motive of this study which was on the isolation, antibiotic profile and glyphosate utilization of Pseudomonas aeruginosa in rice paddy soils from Abaomege in Onicha and Agubia in Ikwo Local Government Areas of Ebonyi State, Nigeria. The study isolated, characterized, and evaluated the antibiotic susceptibility and glyphosate utilization profiles of Pseudomonas aeruginosa from the study sites. Soil samples were collected below the soil surface using soil auger, transported to the laboratory using sterile polythene bag, cultured on a CLED medium and growth of Pseudomonas aeruginosa observed. The organism was isolated and sub-cultured to obtain a pure culture, identified using conventional bacteriological techniques and API system. The antibiotic susceptibility testing was carried out and the isolates were found to be susceptible to Pefloxacin and Ciprofloxacin and resistant to Nalidixic acid. The growth of the isolates in a glyphosate herbicide treated soil was observed. Increased growth was observed after day 3 to day 7 in the sample with the highest concentration of herbicide, while it decreased in the other samples after day 3. Growth rate was significant (p < 0.05) at different time and herbicide concentrations. The study revealed that the infectious agent, P. aeruginosa is common in glyphosate treated soil and its infections can be treated with range of antibiotics. It also showed that the organism can be good in the bioremediation of Glyphosate contaminated soil.
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