Pollution Research Paper

PREVALENCE OF INTESTINAL PARASITES AND DIFFERENTIATE ENTAMOEBA HISTOLYTICA/ E.DISPAR/ E. MOSHKOVSKII IN REFERRED PATIENTS TO THE MEDICAL CENTERS OF ZAHEDAN, IRAN

AFARIN HANAFI, ALIREZA SALIMI KHORASHAD,, ALI HAGHIGHI, HAMED KIANI AND MOHAMMAD JAFARI MODREK

Abstract

Intestinal parasites infections (IPIs) tend to cause serious problems such as abdominal pain, fever, diarrhea and even lead to disability and death in the worldwide. These infections can reduce the quality of life in communities affected. This cross sectional study was conducted with randomized sampling in 2015- 2016 on 1550 patients with abnormal gastrointestinal symptoms (GI) symptoms who had referred to Bu Ali Hospital Laboratory in Zahedan City. All specimens were examined wet mount and lugol staining, formalin-ether concentration and trichrome staining. Nested-PCR was used for detection of amoeba species. Finally, Data were analysis by Statistical Package for the Social Sciences software. Overall, 11.16% (173/ 1550) of the patients studied were infected by IPs. Among them 151 (9.74%) patients infected by single parasite and 22 (1.41%) had poly parasitism. Proportion of protozoan and helminthes parasites was (161 of 173) and (12 of 173) respectively. Prevalence of IPIs in winter (25.8%) was significantly higher than autumn (13.3%) and summer season (7.9%). significantly correlation observed among IPs by season (P = 0.001). According to molecular analysis, all of the Entamoeba histolytica/E.dispar/E. moshkovskii microscopic positive samples were nonpathogenic species (Entamoeba dispar). Prevalence of IPIs in Zahedan City was very decreased in comparison with to past decade, but with the spread of the pathogenic parasites in this area, we should not neglect hygiene measures and control. Giardiasis is still prevalent in this area. E. dispar is the predominant species in human among 3 species like Amoeba (E.histolytica/ E. dispar/ E. moshkovskii). Age and climate (variation season) are two chief predictors for occurrence of IPIs in this region.