Ecology, Environment and Conservation Paper

Vol 24, Issue 1 2018; Page No.(240-245)

SEABUCKTHORN RHIZOBACTERIA PRODUCE ACC DEAMINASE AND EXHIBIT PGPR TRAITS

Pooja Bhadrecha, Madhu Bala, Joginder Singh, Neeta Raj Sharma1and Manoj Kumar

Abstract

Hippophae rhamnoides L. is a wonder-plant with enormous range of health benefits and is astonished with its abilities to survive in the harsh climatic conditions of Leh, India. In the current study, we made an attempt to intrude in its rhizosphere to divulge the microbiome present there. We were able to incur a diverse range of bacterial strains isolated from the rhizospheric soil and plant roots, identified by 16S rRNA. To understand the role played by this wide range of bacteria dwelling in its rhizosphere vicinity, PGPR tests like phosphates solubilization, HCN production and siderophore production were conducted. Out of isolated 17 rhizobacterial isolates, 6 could solubilize phosphate, 10 produced HCN and 10 were found to produce siderophores, in laboratory conditions. Further, these isolates were examined for production of the very essential enzyme ACC deaminase. The qualitative and quantitative analysis revealed that 13 out of these 17 rhizobacteria were capable of producing ACC deaminase. Tukey’s test was applied for statistical analysis of divergence in the ACC enzyme activity.

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