Ecology, Environment and Conservation Paper

IDENTIFICATION AND ISOLATION OF FUNGI INDIGENUS ON SPONTANEOUS FERMENTATION CORN FLOUR BISI 18

Andi Sukainah, Eva Johannes and Reski Praja Putra

Abstract

Modification of corn flour using spontaneous fermentation methods were deemed to be flawed in that type of microbe that lives can be varied and difficult to control. The fermentation process needs to be controlled so that the resulting higher quality flour, one of the efforts is the utilization of microbial isolates indigenus as starter cultures. This study aims to determine the microbes involved in spontaneous fermentation of corn as well as the isolation and identification of fungi indigenus. Corn milling was added water that has been cooked in the ratio 1: 2 (b / v) spontaneously fermented in a fermentation tank clean for 48 hours in microaerophilic. The calculation of the number of microbes performed to determine the indigenus microbes (fungi, yeasts, bacteria and lactic acid bacteria) involved during the process of spontaneous fermentation using media PCA, PDA, NA, and MRSA. In addition, the total acid titration (%) and the pH of the fermentation liquid was also measured. Calculations and analysis was conducted on fermentation time interval of 0, 3, 6, 24, 27, 30, and 48 hours. The results showed that at the beginning of fermentation, which is 0 to 3 hours (adaptation phase), a microbe that was instrumental is mold, after fermentation entered the exponential phase and stationary, i.e. 6 to 48 hours, the fermentation process is dominated by the growth of yeasts, bacteria, and bacteria lactic acid which causes increased levels of total acid titration until 0.38% and the pH value becomes 3.970. In this phase, the mold growth decreased. The result of the isolation and identification of fungi using slide culture techniques are found 7 species of mold that are involved in the process of spontaneous fermentation of corn Bisi 18, namely Aspergillus fumigatus, A. flavus, 3 types of classified Aspergillussp, Cunninghamella elegans, dan Dendryphio psisatra.