FATTY ACID METHYL ESTERS PROFILE OF EDWARDSIELLA TARDA ISOLATED FROM DISEASED AFRICAN CATFISH CLARIAS GARIEPINUS (BURCHELL 1822)HARRESH ADIKESAVALU H.M. AND T. JAWAHAR ABRAHAM
The cellular fatty acid methyl esters (FAME) profile of Edwardsiella tarda strain CGH9 isolated from diseased African catfish Clarias gariepinus in West Bengal, India was determined by gas-liquid chromatography. The FAME profile revealed a total of 23 fatty acids with the dominance of saturated fatty acids (47.8%) and unsaturated fatty acids (42.35%). Cyclopropane fatty acids (8.75%), hydroxylated fatty acids (8.44%) and branched chain fatty acids (0.20%) were also present. Of the 23 fatty acids, six fatty acids such as 16:0 (hexadecanoic acid), 14:0 (tetradecanoic acid), 16:1 ω6c and/or16:1 ω7c (cis-6-hexadecenoic acid and/or cis-7-hexadecenoic acid), 18:1 ω6c and/or 18:1 ω7c (cis-6-octadecanoic acid and/or cis-7-octadecanoic acid), 14:0 3OH and/or 16:1 isoI (3-hydroxylatedtetradecanoic acid and/or hexadecenoic acid isomer I) and 17:0 cyclo (cis-9,10-methylene hexadecanoic acid) were present at a level above 1%. The FAME profile of the strain CGH9 were almost similar to those of the standard E. tarda strain ATCC 15947. The phenotypic characteristics and FAME analysis, thus, confirmed the strain CGH9 as E. tarda. As the FAME analysis is rapid and accurate, this method can be used for the rapid identification of fish bacterial pathogens in conjunction with molecular methods.
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